Product Usage Information
Application | Dilution |
---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Immunofluorescence (Frozen) | 1:100 - 1:400 |
Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #93473.
Specificity / Sensitivity
β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. Depsite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, β-Actin (13E5) Rabbit mAb #4970 does not cross-react with cytoplasmic γ-actin, or any other actin isoforms.
仅限科研使用
Applications:WB, W-S, IHC-P, IF-F, IF-IC, FC-FP
Source/Isotype:Rabbit IgG